Abstract
Unmasking tumor heterogeneity and clonal evolution by single-cell analysis
Highlights
A single cell is the ultimate denominator of a multicellular organism
In the progression of cancer, a single cell begins its journey to evolve into a malignant tumor cell and forms distinct subpopulations leading to intratumoral heterogeneity (ITH)
A novel technique termed Drop-seq uses the microfluidic chamber to isolate single cells followed by labeling RNA of individual cells with a different barcode, allowing pooling of cDNA during sequencing thereby greatly improving the multiplexing efficiency[100]
Summary
A single cell is the ultimate denominator of a multicellular organism. In the progression of cancer, a single cell begins its journey to evolve into a malignant tumor cell and forms distinct subpopulations leading to intratumoral heterogeneity (ITH). By isolating and sequencing single CTCs in the blood, it is possible to measure somatic mutations that are present at both the primary and metastatic tumor sites without performing an invasive core biopsy[59,60]. A novel technique termed Drop-seq uses the microfluidic chamber to isolate single cells followed by labeling RNA of individual cells with a different barcode, allowing pooling of cDNA during sequencing thereby greatly improving the multiplexing efficiency[100].
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