Unlocking the potential of SiO2 and CeO2 nanoparticles for arsenic mitigation in Vigna mungo L. Hepper (Blackgram).

Abstract

In this study, the interaction effects of NaAsO2 (1 and 5μM), SiO2 NPs (10 and 100mg/L) and CeO2 NPs (10 and 100mg/L) were assessed in Vigna mungo (Blackgram). The treatment of NaAsO2, SiO2, CeO2-NPs and combinations of NPs & As were applied to blackgram plants under hydroponic conditions. After its application, the morpho-physiological, antioxidant activity, and phytochemical study were evaluated. At 10 and 100mg/L of SiO2 and CeO2-NPs, there was an increase in antioxidative enzymatic activity (p < 0.05) and reactive oxygen species (ROS). However, substantial ROS accumulation was observed at 1 and 5μM NaAsO2 and 100mg/L SiO2 NPs (p < 0.05). Additionally, at such concentrations, there is a substantial reduction in photosynthetic pigments, nitrogen fixation, chlorosis, and plant development when compared to controls (p < 0.05). The combination of SiO2 and CeO2 NPs (10 and 100mg/L) with NaAsO2 decreased superoxide radical and hydrogen peroxide and improved SOD, CAT, APX, GR, and chlorophyll pigments (p < 0.05). Further FTIR results were evaluated for documenting elemental and phytochemical analysis.