Abstract
Flowering is a crucial process in the life cycle of most plants as it is essential for the reproductive success and genetic diversity of the species. There are situations in which breeders want to expedite, delay, or prevent flowering, for example, to shorten or prolong vegetative growth, to prevent unwanted pollination, to reduce the risk of diseases or pests, or to modify the plant’s phenotypes. This review aims to provide an overview of the current state of knowledge to use CRISPR/Cas9, a powerful genome-editing technology to modify specific DNA sequences related to flowering induction. We discuss the underlying molecular mechanisms governing the regulation of the photoperiod, autonomous, vernalization, hormonal, sugar, aging, and temperature signal pathways regulating the flowering time. In addition, we are investigating the most effective strategies for nominating target genes. Furthermore, we have collected a dataset showing successful applications of CRISPR technology to accelerate flowering in several plant species from 2015 up to date. Finally, we explore the opportunities and challenges of using the potential of CRISPR technology in flowering time engineering.
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