University of Wisconsin solution versus modified collins solution for canine heart preservation: An experimental study

Abstract

We investigated the efficacy of University of Wisconsin (UW) solution for its ability to preserve hearts for 12 hours as compared with the results with modified Collins (MC) solution. After orthotopic transplantation, hearts stored in the UW solution (68%) showed significantly (p<0.05) better recovery of maximum rate of increase of left ventricular pressure than those stored in the MC solution (41%) (MC group: n=6, UW group: n=7). The serum CPK values 2 hours after reperfusion were significantly (p<0.01) higher in the MC group (6528 IU/L) than in the UW group (2021 IU/L). Myocardial adenosine triphosphate (ATP) levels were measured using phosphorus 31 nuclear magnetic resonance spectroscopy during 12 hours of cold storage (MC group: n=5, UW group: n=4). ATP levels continued to decrease throughout the storage, and fell to 39% (MC group) and 23% (UW group) of the control after 12 hours with no significant difference. Significant differences between the two groups were present in water content before (MC group: 77.6%, UW group: 75.8%;p<0.05) and after (MC group: 77.6%, UW group: 75.8%;p<0.025) 12 hours of storage (MC group:n=6, UW group:n=5). These results demonstrated that the UW solution was superior to the MC solution when used to preserve hearts for 12 hours.