Universal stress protein-like gene from mulberry enhances abiotic stress tolerance in Escherichia coli and transgenic tobacco cells.

Abstract

Universal stress proteins (USPs) are a conserved group of proteins initially identified and characterized in bacteria. USPs are induced under multiple stresses, and are important for stress acclimation. We cloned a USP-like gene designated as MaUSP1-like from mulberry and expressed in bacteria and tobacco to examine its relevance in abiotic stress tolerance. Escherichia coli and tobacco cells expressing MaUSP1-like gene were exposed to different abiotic stresses, and cell survival and growth was recorded to assess the stress effects. MaUSP1-like gene conferred tolerance to E.coli cells under NaCl-induced salt stress, PEG8000-induced desiccation stress, cadmium chloride-induced heavy metal stress, and heat stress. Overexpression of MaUSP1-like sustained cell division and growth in tobacco cells under salt stress. The results demonstrate that MaUSP1-like gene is capable of conferring cellular level tolerance in both prokaryotic and eukaryotic systems, under abiotic stress. The finding opened up an option to argue that maintenance of cellular level tolerance is crucial for sustenance of growth under stress and cellular level tolerance can be improved by overexpressing genes like USPs.