Abstract
Technologies for the rapid and accurate diagnosis of infectious diseases are urgently needed. There were many methods developed for the diagnostic purpose, including microarrays, biosensors, multiplex PCR, combined PCR with mass spectrometry, capillary electrophoresis, and lab-on-chip tests, which have a major impact on the rapid diagnosis of infectious diseases. Each of the above methods has several unsolvable shortcomings, such as complexity and time-consuming operation, high cost requiring special equipment (electrospray ionization/mass spectrometry). Therefore, use of these methods has been limited in clinical practice. In the present article, we focus on a new method that couples broad-range PCR amplification with high-resolution melting analysis, which serves as a novel approach for molecular diagnosis of infectious diseases. The strategies and applications of this method are discussed.
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