United Forces of Botanical Oils: Efficacy of Neem and Karanja Oil against Colorado Potato Beetle under Laboratory Conditions.

Kateřina Kovaříková,Roman Pavela

doi:10.3390/plants8120608

Abstract

Neem and karanja oil are the most promising botanical insecticides in crop protection nowadays. Given that information about the insecticidal abilities of these oils is lacking, the aim was to explore the effects of neem and karanja oil binary mixtures. The insecticidal activity of NeemAzal T/S (Trifolio-M GmbH, Lahnau, Germany) (neem oil), Rock Effect (Agro CS a.s., Česká Skalice, Czech Republic) (karanja oil), and their binary mixes (at 1:1, 1:2, and 2:1 volume ratios) against the larvae of the Colorado potato beetle (CPB; Leptinotarsa decemlineata) was studied. In our bioassays, a synergistic effect of the mixtures, which was dose-dependent, was observed for the first time against this pest. The most effective blend was the 1:1 ratio. Its efficacy was more or less the same as, or even greater than, the neem oil alone. The LC50 of neem oil two days after application was (0.075 g·L−1) and the LC50 of the mixture was (0.065 g·L−1). The LC50 of karanja oil was (0.582 g·L−1), which was much higher than the LC50 of neem oil. The LC90 of neem oil five days after application was (0.105 g·L−1) and the LC90 of the mixture was (0.037 g·L−1). The LC90 of karanja oil was (1.032 g·L−1). The results demonstrate that it is possible to lower the doses of both oils and get improved efficacy against CPB larvae; nevertheless, further verification of the results in field conditions is necessary.

Highlights

The Colorado potato beetle (CPB), (Leptinotarsa decemlineata (Say, 1824), Coleoptera: Chrysomelidae) is one of the most important potato pests
From the LC50 (LC90 ) of Rock Effect (Agro CS a.s., Česká Skalice, Czech Republic) (0.582 g·L−1 ) and NeemAzal T/S (Trifolio-M GmbH, Lahnau, Germany) (0.075 g·L−1 ), it is obvious that karanja oil by itself is significantly less effective against larvae of Leptinotarsa decemlineata in comparison with neem oil
This experiment involved testing the insecticidal activity of NeemAzal T/S (Trifolio-M GmbH, Lahnau, Germany), Rock Effect (Agro CS a.s., Česká Skalice, Czech Republic), and three binary mixtures

Summary

Introduction

The Colorado potato beetle (CPB), (Leptinotarsa decemlineata (Say, 1824), Coleoptera: Chrysomelidae) is one of the most important potato pests. This species is native to North America, from where it has gradually spread across Europe and Asia [1] alongside the expansion of potato cultivation. Mailloux and Bostanian [4] stated that a measure based on the level of defoliation is better than one based on pest abundance. These levels were estimated, for example, by Zehnder et al [5] at 20%

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