Unitary Response of Solvatochromic Dye to Pulse Excitation in Lipid and Cell Membranes

Abstract

The existence of acoustic pulse propagation in lipid monolayers at the air-water interface is well known. These pulses are controlled by the thermodynamic state of the lipid membrane. Nevertheless, the role of acoustic pulses for intra- and inter-cellular communication is still a matter of debate. Herein, we used the dye di-4-ANEPPDHQ, which is known to be sensitive to the physical state and transmembrane potential of membranes, in order to gain insights into compression waves in lipid-based membrane interfaces. The dye was incorporated into lipid monolayers made of phosphatidylserine or phosphatidylcholine at the air-water-interface. A significant blue shift of the emission spectrum was detected when the state of the monolayer was changed from the liquid-expanded (LE) to the liquid-condensed (LC) phase. This "transition sensitivity" of di-4-ANEPPDHQ was generalized in experiments with the bulk solvent dimethyl sulfoxide (DMSO). Upon crystallization of solvent, the emission spectrum also underwent a blue shift. During compression pulses in lipid monolayers, a significant fluorescence response was only observed when the main transition is crossed. The optical signature of these waves─in terms of sign and magnitude─was identical to the response of di-4-ANEPPDHQ during action potentials in neurons and excitable plant cells. These findings corroborated the suggestion that action potentials are nonlinear state changes that propagate in the cell membrane.