Abstract
A study was made of eight strains of tobacco mosaic virus. It was found possible to make the comparison of average particle length on duodisperse preparations. The duodisperse preparations exhibited two characteristic peaks in the analytical ultracentrifuge. Of these peaks, one corresponded to the particle length (monomers) and the other most probably to particles having twice the particle length (dimers). Smaller quantities of particles if intermediary lengths were also present in these preparations. The extinction angle-gradient curves of all strains could be well represented by Sadron's equation using a double term numerator and denominator. The agreement in extinction angle gradient curves computed from electron micrographs and direct observation in the flow birefringence apparatus was very good. All eight strains have lengths of 3,350 ± 250 A. This value is in excellent agreement with recently reported electric birefringence values and our calculated and correct electron microscope values. The strains have markedly different optical factors, and this was discussed in the light of the possibility that such differences in principal indices of refraction could be caused by differences in amino acid and/or nucleotide sequences.
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