Unipolar microtubule array is directly involved in nurse cell-oocyte transport.

Abstract

The telotrophic ovariole of Rhodnius prolixus is richly endowed with microtubules (MTs). An extensive, stable array of MTs packs the trophic core and trophic cords which link the nurse cell compartments to the growing oocytes. This system is excellent to study MT-based transport as the MTs are believed to play a role in transport of nurse cell-produced mitochondria, ribosomes, and mRNAs to the oocytes. We investigated MT polarity and molecular MT motors in this unidirectional transport system. Hook decoration revealed that the MTs of the trophic core and cords have their plus (+) ends in the tropharium and minus (-) ends in the oocytes. Video differential interference optics (DIC) microscopy showed that vesicle transport was saltatory, ATP-dependent, and had an average velocity of 0.77 micron/sec toward the oocyte. Transport was sensitive to 2 mM N-ethylmaleimide (NEM) and 50 microM vanadate and resistant to 1 mM 5'-adenylylimidodiphosphate (AMP-PNP) and 5 microM vanadate. We report that the unipolar, acetylated trophic cord MTs play a direct role in nurse cell-oocyte transport via a cytoplasmic dynein-like retrograde motor.