UniPep - a database for human N-linked glycosites: a resource for biomarker discovery

Hui Zhang,Andrew Keller,Hookeun Lee,Jimmy Eng,Ruedi Aebersold,Richard D Smith,Kelly Cooke,Yong Zhou,Bernd Wollscheid,Richard Bonneau,Paul Loriaux,Elaine R Peskind,Jing Zhang,Ning Zhang,David Campbell,Pat Moss,Eugene C Yi

doi:10.1186/gb-2006-7-8-r73

Abstract

UniPep, a database of human N-linked glycosites is presented as a resource for biomarker discovery

Highlights

It is generally understood that variations in an individual's genetic background and physiologic state give rise to alterations in the person's plasma protein profile. (For the purposes of this report, the terms 'serum' and 'plasma' are used interchangeably.) Of particular interest are those changes that reflect important processes in specific organs or tissues, such as the early onset of pathologic processes or the response to pharmacologic intervention
Careful analysis of the results produced by such studies has indicated the restricted dynamic range of the analytical methods used as a main limitation [15]
To display all the theoretical N-linked glycosites in the human International Protein Index (IPI) database and to relate them to the N-linked glycosites that were experimentally observed by mass spectrometric analysis, we developed the UniPep database and web interface [21]

Summary

Introduction

It is generally understood that variations in an individual's genetic background and physiologic state give rise to alterations in the person's plasma protein profile. (For the purposes of this report, the terms 'serum' and 'plasma' are used interchangeably.) Of particular interest are those changes that reflect important processes in specific organs or tissues, such as the early onset of pathologic processes or the response to pharmacologic intervention. The limited number of N-linked glycosites in the human proteome suggests that all or at least the majority of these peptides can be identified if respective data from different experiments and laboratories are integrated into a single comprehensive database.

Results

Conclusion