Abstract
Uniform-sized macroporous polymer-based separation media were prepared through a typical two-step swelling method utilizing vinyl methacrylate or other ordinary cross-linking agents to investigate their properties as packing materials in HPLC. A simple and less hydrophobic cross-linking agent, vinyl methacrylate, afforded stable macroporous packing materials unexpectedly affording the largest retention volume towards hydrophobic solutes in reversed-phase liquid chromatography with good column efficiency. The retention ability towards seven halogenated organic solvents from chloroform to tetrachloroethylene was found to be much higher than that with a typical hydrophobic polymer-based packing material, poly(styrene-divinylbenzene) particles, and also a typical C 18 silica-based hydrophobic station phase.
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