Abstract
Peptoids that are helical and partially water soluble have been shown to self-assemble into microspheres when the peptoid solution is dried on a silicon substrate. Such microsphere coatings have great potential for use in biosensor technologies, specifically to increase the surface area for binding. However, in order to be useful, the peptoids must consistently form uniform coatings. In this study we investigated the effects of various coating protocol parameters on the uniformity of the resulting peptoid microsphere coatings, including (i) solvent, (ii) administration technique, and (iii) drying environment. In addition, we investigated the robustness of the coatings as well as the potential for using a glass substrate. These studies show that uniform, robust peptoid microsphere coatings can be formed using protic solvents, a full coverage administration technique, and drying in open air on silicon or glass substrates.
Highlights
The pursuit of an efficient, non-invasive, and economical tool for the early detection of complex diseases, such as cancer, has led to the development of sophisticated biosensor technologies for protein analysis
Previous studies in our lab have shown that helical peptoids that are partially soluble in water form microspheres when dried from aqueous/organic solutions [13]
Previous work from our lab showed that helical peptoids with partial water solubility reproducibly self-assemble into microspheres, but uniform coatings were not consistently achieved [13]
Summary
The pursuit of an efficient, non-invasive, and economical tool for the early detection of complex diseases, such as cancer, has led to the development of sophisticated biosensor technologies for protein analysis. Coatings 2013, 3 area for binding, (ii) resistance to biofouling, and (iii) flexible synthesis that allows for incorporation of a variety of chemistries. Such a coating is possible using self-assembled poly-N-substituted glycine (or peptoid) microspheres. Peptoids have great potential for use as biosensor coatings due to their ability to increase surface area through supramolecular assemblies, their ease of synthesis, and the numerous side chain chemistries available. We found that protic solvents, drying at standard conditions, and full coating of the slide reproducibly resulted in uniform coatings Utilizing these protocols, the peptoid microsphere coatings are robust and can be created on glass and silicon substrates
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