Unfractionated Heparin Promotes Osteoclast Formation in Vitro by Inhibiting Osteoprotegerin Activity.

Binghan Li,Yuqing Chen,Minghui Zhao,Li Zuo,Dan Lu

doi:10.3390/ijms17040613

Abstract

Heparin has been proven to enhance bone resorption and induce bone loss. Since osteoclasts play a pivotal role in bone resorption, the effect of heparin on osteoclastogenesis needs to be clarified. Since osteocytes are the key modulator during osteoclastogenesis, we evaluated heparin’s effect on osteoclastogenesis in vitro by co-culturing an osteocyte cell line (MLO-Y4) and pre-osteoclasts (RAW264.7). In this co-culture system, heparin enhanced osteoclastogenesis and osteoclastic bone resorption while having no influence on the production of RANKL (receptor activator of NFκB ligand), M-CSF (macrophage colony-stimulating factor), and OPG (osteoprotegerin), which are three main regulatory factors derived from osteocytes. According to previous studies, heparin could bind specifically to OPG and inhibit its activity, so we hypothesized that this might be a possible mechanism of heparin activity. To test this hypothesis, osteoclastogenesis was induced using recombinant RANKL or MLO-Y4 supernatant. We found that heparin has no effect on RANKL-induced osteoclastogenesis (contains no OPG). However, after incubation with OPG, the capacity of MLO-Y4 supernatant for supporting osteoclast formation was increased. This effect disappeared after OPG was neutralized and reappeared after OPG was replenished. These results strongly suggest that heparin promotes osteocyte-modulated osteoclastogenesis in vitro, at least partially, through inhibiting OPG activity.

Highlights

Heparin, a highly-sulfated glycosaminoglycan, is an anticoagulant that is widely used in various thrombotic diseases and during hemodialysis [1]
Our previous study of rats with chronic kidney disease (CKD) confirmed that heparin treatment could induce bone loss, and further suggested that increased osteoclastic bone resorption may be involved in this effect [3]
Since RANKL is produced by osteoblasts and osteocytes, both could support osteoclast formation [6,7,8]

Summary

Introduction

A highly-sulfated glycosaminoglycan, is an anticoagulant that is widely used in various thrombotic diseases and during hemodialysis [1]. Its administration can induce bone volume loss and decreased bone density [2]. This adverse effect is critical for patients undergoing long-term heparin therapy, such as maintenance hemodialysis patients. Our previous study of rats with chronic kidney disease (CKD) confirmed that heparin treatment could induce bone loss, and further suggested that increased osteoclastic bone resorption may be involved in this effect [3]. We aimed to investigate the effects of heparin on osteoclast formation and activity in vitro. Receptor activator of NFκB ligand (RANKL; called TRANCE, ODF, and OPGL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) are the most important factors regulating osteoclast formation [4]. Since RANKL is produced by osteoblasts and osteocytes, both could support osteoclast formation [6,7,8]

Objectives

Methods

Results