Abstract
Ultrafiltration is a standard method for separating free T3 and free T4 from serum proteins and protein-bound hormone (1)(2). It is expected that free T3 and free T4 in aqueous solutions will accompany water as water moves across semipermeable membranes, a process that results in equal concentrations of free T3 and free T4 in the aqueous solutions on opposite sides of semipermeable membranes after ultrafiltration. The movements of free thyroid hormones through semipermeable membranes have not been compared to the movement of water, nor have the concentrations of free hormones on opposite sides been determined in the absence of hormone-binding proteins. We report experiments with 4 different ultrafiltration devices to determine the movements of 3H2O, 125I-T3, and 125I-T4 across semipermeable membranes in a biologically relevant protein-free aqueous solution. A pool of normal human serum was obtained (Equitech-Bio). Serum thyroxine-binding globulin, transthyretin, albumin, total protein, total T4, dialyzable (free) T4, and thyroid-stimulating hormone were within their respective reference intervals (Quest Diagnostics). Serum pH was controlled at 7.4 by adding HEPES …
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