Abstract
AbstractA new method of immobilization of low‐molecular‐weight compounds or antibodies on nylon 66 was developed. Secondary amide groups of nylon 66 were converted into secondary imidate groups by O‐alkylation with sulfuric acid and methanol. The methoxycarbonimidoyl groups react with amino compounds to produce stable amidine links. Antibodies and several low‐molecular‐weight compounds (amino acids) were immobilized by covalent linkage on nylon balls. Fixed antibodies were used in solid phase enzyme linked immunoassay, i.e. applied for the detection of K99+ Escherichia Coli in calves.
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