Abstract
Nanofluidic controls of single DNA molecules have provided a new approach of dynamically stretching large DNA molecules. Stretched DNA molecules enables single-molecule schemes aimed at the acquisition of sequence information. Also, nanoconfined DNA molecules provide opportunities to understand mechanistic details that used to be only plausible in theoretical considerations. Here we present the longest DNA molecules stretched in nanochannel ever reported: 20.0 μm out of 21. 8 μm of YOYO-1 intercalated lambda DNA which is 92% of polymer's full contour length in PDMS nanoslits. In addition, we measure these elongations in various dimensions and reduced ionic strength to facilitate DNA elongation. Finally we compare our observations with theoretical predictions recently developed.
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