Abstract
Despite substantial researches on the phytochemical profile of Geranium under various circumstances, little is known about its temperature-stress management strategies and responsible key genes. The involvement of PgFPPS and PgGPPS in temperature-stress management was investigated via in-silico studies, and gene expression patterns in conjunction with other metabolic genes, transcription factors and trichome-related genes. Docking studies of PgFPPS and PgGPPS revealed the active site and key amino acids implicated in substrate bindings. Further, Geranium plants were grown under a variety of temperature-stress circumstances to examine the temperature-stress management strategies at physiological, biochemical, and molecular level. The plants were physiologically and biochemically (including enzymatic and non-enzymatic antioxidant machinery) profiled. Gene expression analysis of PgFPPS, PgGPPS, PgDXS, PgDXR, PgHMGS, PgHMGR, PgGES, PgEDS, PgLS and transcription factors (PgMYB, PgWRKY, and PgERF) revealed their regulatory role in temperature-stress management. The GC and FT-IR analyses conferred the variation in its phytoconstituents (EOs) composition. The metabolite profiling led to the conclusion that temperature change was directly/indirectly correlated with oil yield and composition. Trichomes underwent a variation in density under temperature stress which was confirmed by gene expression analysis of trichome-related genes (PgCPC, PgGL1, PgTT8 and PgTRY). These genes was found to be involved in metabolite flux along with TFs. To the best of our knowledge, this is the first report that provide interesting insights into the role of important metabolic genes, transcription factors, trichome-related genes in relation to essential oil content under temperature-stress management strategies.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
