Abstract
A detailed knowledge of cell wall heterogeneity and complexity is crucial for understanding plant growth and development. One key challenge is to establish links between polysaccharide-rich cell walls and their phenotypic characteristics. It is of particular interest for some plant material, like cotton fibers, which are of both biological and industrial importance. To this end, we attempted to study cotton fiber characteristics together with glycan arrays using regression based approaches. Taking advantage of the comprehensive microarray polymer profiling technique (CoMPP), 32 cotton lines from different cotton species were studied. The glycan array was generated by sequential extraction of cell wall polysaccharides from mature cotton fibers and screening samples against eleven extensively characterized cell wall probes. Also, phenotypic characteristics of cotton fibers such as length, strength, elongation and micronaire were measured. The relationship between the two datasets was established in an integrative manner using linear regression methods. In the conducted analysis, we demonstrated the usefulness of regression based approaches in establishing a relationship between glycan measurements and phenotypic traits. In addition, the analysis also identified specific polysaccharides which may play a major role during fiber development for the final fiber characteristics. Three different regression methods identified a negative correlation between micronaire and the xyloglucan and homogalacturonan probes. Moreover, homogalacturonan and callose were shown to be significant predictors for fiber length. The role of these polysaccharides was already pointed out in previous cell wall elongation studies. Additional relationships were predicted for fiber strength and elongation which will need further experimental validation.
Highlights
Cell walls, the key determinant of overall plant growth and development are primarily composed of polysaccharides, namely cellulose, hemicellulose, and pectins, lignin, and structural proteins [1,2]
Standardization of the raw data In this study, we attempted to assess the relationship between the cell wall polysaccharides and the physical fiber properties of mature cotton fibers, the data of which are provided as Table S1 and S2
To make the fiber characteristics dataset compliant to the glycan array, the raw data were jointly standardized using z scores prior to the analysis
Summary
The key determinant of overall plant growth and development are primarily composed of polysaccharides, namely cellulose, hemicellulose, and pectins, lignin, and structural proteins [1,2]. Since the early 1970’s, comparative biochemical analyses revealed that all plant cell walls share several common features. They exhibit diversity with respect to their chemical composition [3,4,5]. Cell walls are structurally complex as they are constantly remodeled and reconstructed during plant growth and development. Biochemical analyses complemented by genetic analyses have identified genes and gene products associated with cell wall synthesis. Understanding the plant cell walls is fundamental to plant sciences and of industrial relevance
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