Understanding the Nature of Variations in Structural Sequences coding for Coronavirus Spike, Envelope, Membrane and Nucleocapsid Proteins of SARS-CoV-2

Abstract

Context: Given the worldwide acceleration of the Coronavirus CoVID-19 pandemic, it now mandates an absolute need for therapeutic remedies or vaccines to contain its further spread and to halt its progress. In this context, our attempt is to find using a novel technique, a possible solution to detect and identify the nature of mutations occurring in the viral genome to design and develop potential agents that might act as vaccines. Objectives: (i) To understand and delineate the nature of variations and mutations in coronaviral genome, and (ii) To use the knowledge thus available for designing putative vaccine element against coronavirus CoVID-19 infection using genomic materials derived from the ORFs and other structural CDS sequences of this virus. Data Sources: The sequence data of coronavirus have been accessed from the NCBI Genbank. Conclusions: This paper reports study of the nature of structural proteins encoded by the genomic sequences and delineates the variations or mutations found to have occured in the most unstable sequences. This might help researchers to design and develope putative candidates against SARS-CoV-2 virus by designing oligopeptides which can be derived from the sequences that codes for structural coronavirus proteins or Open Reading Frames (ORFs) of the novel Coronaviral genome (previously 2019 nCoV).