Understanding the mechanisms of zeolite in inhibiting Pb accumulation in different rice cultivars (Oryza sativa).

Abstract

Zeolite is one of the potential passivating amendments for the immobilization of lead (Pb) in contaminated farmland soils. In this study, pot experiments were carried out to investigate the effects and the mechanisms of zeolite on Pb accumulation in two rice cultivars grown in a slightly Pb-contaminated soil. Results showed that Pb content in grains of Zheyou 18 (ZY-18) decreased by the addition of 6g zeolite kg-1 soil (E6), which can be attributed to the reduction in soil Pb availability, dissolved organic carbon (DOC), water-soluble iron (Fe) and manganese (Mn), and the transfer factor from soil to grain (TFsoil-grain). These reductions were mainly resulting from the significant increase in soil pH, glutathione (GSH), phytochelatins (PCs), and non-protein (NPT) content in rice root, and the decrease in soil redox potential (Eh), due to zeolite addition. Pb content in brown rice of DL-5 was not significantly affected with E6 treatment, whereas it was raised by applying 12g zeolite kg-1 soil (E12). The increase of Pb content of Donglian 5 (DL-5) grains with E12 treatment can be attributed to more Pb uptake by the root, higher Pb transfer factors (TFs) between various parts of rice, and significant decrease in GSH, PCs, and NPT contents in the root. It is concluded that a suitable rate of zeolite addition can immobilize Pb in slightly Pb-contaminated acidic soil. However, the final immobilization effect also depends on rice cultivars.