Understanding the dependence of start-up and stability of aerobic granule on pH from the perspective of adhesion behavior and properties of extracellular polymeric substances

Abstract

The start-up and stability of aerobic granular sludge (AGS) could be greatly influenced by pH variation. The inner core in the aerobic granules provided adhesion sites for microbes by extracellular polymeric substances (EPS) adhesion, the adhesion behavior of EPS and the properties of adhesion layer formed by EPS with pH changes might directly affect the start-up efficiency and stability of AGS. In this study, the adhesion behavior of EPS at an inorganic surface and the viscoelasticity of the EPS adhesion layer with pH variation was investigated by quartz crystal microbalance with dissipation monitoring, and the response of functional groups and intermolecular interactions to pH changes was explored. Based on the interaction energy calculation, it was found that the charge repulsion between substances dominated the interactions between EPS components and between EPS and the surface by regulating protonation and deprotonation of the functional groups of EPS with pH variation. A lower energy barrier between EPS and the surface at a lower pH value could facilitate the adhesion of EPS at the surface, which favored the rapid start-up of AGS. Moreover, the high ratio of both α-helix and intermolecular hydrogen bond at an acid condition could enhance the gel-strength of EPS, which provide AGS the resistance ability against external disturbance. This study revealed the mechanism of the interactions in EPS adhesion process with the variation of pH and provided useful information for a better understanding of the stability of the AGS.