Understanding the crosstalk between ?d T cells and osteoclasts in patients with breast cancer

Abstract

Event Abstract Back to Event Understanding the crosstalk between γδ T cells and osteoclasts in patients with breast cancer Shubhada Chiplunkar1*, Swati P. Phalke1, Vani Parmar2 and Rajendra A. Badwe2 1 ACTREC-TMC, India 2 Tata Memorial Hospital, India Bone metastasis is a common cause of morbidity in breast cancer patients. Aminobisphosphonates are widely used in treatment of bone metastasis and skeletal disorders. Aminobisphosphonates increase endogenous pool of isopentenyl pyrophosphate (IPP) of mevalonate pathway in tumor cells, which acts as potent antigen for γδ T cells. γδ T cells are unique subsets of T-lymphocytes that are activated by aminobisphosphonates and phosphoantigens (bromohydrin pyrophosphate, IPP). The present study aims at understanding the crosstalk between γδ T cells and osteoclasts in patients with breast cancer metastasis. Multicolor flow cytometry showed higher expression of RANKL on γδ T cells of breast cancer patients. Stimulation with aminobisphosphonate (zoledronate) increased RANKL expression on γδ T cells. Osteoclasts generated from CD14+ monocytes of breast cancer patients and healthy individuals in the presence of zoledronate activated γδ T cells showed reduced osteoclastogenesis as analysed by 23c6 mAb (CD51/61) and DAPI staining. Osteoclasts generated in the presence of cell free supernatants of activated γδ T cells showed reduced resorption area (pits) on Osteoclast Activity Assay Substrate. Cytokine analysis of activated γδ T cells showed presence of IFN-γ, antiosteoclastogenic cytokine. Zoledronate activated γδ T cells have high propensity to migrate to bone and inhibit osteoclastogenesis. Aminobisphosphonate activated γδ T cell based immunotherapy would be a promising approach to treat bone metastasis in breast cancer. Keywords: Aminobisphosphonates, γδ T cells, Osteoclasts, bone metastasis, breast cancer Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Chiplunkar S, Phalke SP, Parmar V and Badwe RA (2013). Understanding the crosstalk between γδ T cells and osteoclasts in patients with breast cancer. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01017 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Shubhada Chiplunkar, ACTREC-TMC, Navi Mumbai, Maharashtra, 410210, India, schiplunkar@actrec.gov.in Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Shubhada Chiplunkar Swati P Phalke Vani Parmar Rajendra A Badwe Google Shubhada Chiplunkar Swati P Phalke Vani Parmar Rajendra A Badwe Google Scholar Shubhada Chiplunkar Swati P Phalke Vani Parmar Rajendra A Badwe PubMed Shubhada Chiplunkar Swati P Phalke Vani Parmar Rajendra A Badwe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.