Abstract
Background Glycolysis is closely associated with tumor progression, but the roles of lncRNAs in glycolysis have not been comprehensively investigated in lung adenocarcinoma (LUAD). This study is aimed at studying the possible mechanisms of glycolysis-related lncRNAs in tumor development and providing a guidance for targeted therapy. Methods Unsupervised consensus clustering was used to identify molecular subtypes. Gene enrichment analysis was applied to screen important pathways involved in tumor progression. A series of immune analysis was performed to assess immune infiltration. Critical transcription factors (TFs) interacting with lncRNAs were selected by Pearson correlation analysis. A first-order partial correlation analysis was implemented to identify critical lncRNAs with prognostic significance. Results Three molecular subtypes (C1, C2, and C3) were identified with distinct overall survival. Three subtypes showed differential immune infiltration, and C3 subtype was the optimal for immunotherapy treatment. Ten lncRNA-TF pairs among four glycolysis-related lncRNAs (FTX, LINC00472, PSMA3-AS1, and SNHG14) and six TFs (FOXP1, SP1, MYC, FOXM1, HIF1A, and FOS) were involved in tumor progression. We identified four critical glycolysis-related lncRNAs significantly associated with prognosis. Conclusions This study identified three molecular subtypes that could guide personalized therapy. The four-lncRNA prognostic model can serve as an indicator for predicting prognosis or early screening of lung adenocarcinoma patients. The current results improve the understanding of the relation between lncRNAs and glycolysis.
Highlights
Lung cancer is a leading cause of cancer death worldwide, and lung adenocarcinoma (LUAD) is the most common primary lung cancer
Glycolysis is closely associated with tumor progression, but the roles of long noncoding RNAs (lncRNAs) in glycolysis have not been comprehensively investigated in lung adenocarcinoma (LUAD)
A total of 3940 lncRNAs in TCGA-LUAD dataset, 379 in GSE31210, and 586 in GSE72094 were identified to be associated with glycolysis activity (Figure 2(a)). 37 of the lncRNAs coexisted in all three datasets, and they were selected for further analysis
Summary
Lung cancer is a leading cause of cancer death worldwide, and lung adenocarcinoma (LUAD) is the most common primary lung cancer. An early screening and diagnosis of lung cancer is currently a great challenged. Glycolysis is closely associated with tumor progression, but the roles of lncRNAs in glycolysis have not been comprehensively investigated in lung adenocarcinoma (LUAD). This study is aimed at studying the possible mechanisms of glycolysis-related lncRNAs in tumor development and providing a guidance for targeted therapy. Gene enrichment analysis was applied to screen important pathways involved in tumor progression. Critical transcription factors (TFs) interacting with lncRNAs were selected by Pearson correlation analysis. A first-order partial correlation analysis was implemented to identify critical lncRNAs with prognostic significance. We identified four critical glycolysis-related lncRNAs significantly associated with prognosis. The four-lncRNA prognostic model can serve as an indicator for predicting prognosis or early screening of lung adenocarcinoma patients. The current results improve the understanding of the relation between lncRNAs and glycolysis
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have