Abstract
BackgroundStreptococcus suis serotype 2 (S. suis 2) is an important pathogen of pigs. S suis 2 infections have high mortality rates and are characterized by meningitis, septicemia and pneumonia. S. suis 2 is also an emerging zoonotic agent and can infect humans that are exposed to pigs or their by-products. To increase our knowledge of the pathogenesis of meningitis, septicemia and pneumonia in pigs caused by S. suis 2, we profiled the response of peripheral blood mononuclear cells (PBMC), brain and lung tissues to infection with S. suis 2 strain SC19 using the Affymetrix Porcine Genome Array.ResultsA total of 3,002 differentially expressed transcripts were identified in the three tissues, including 417 unique genes in brain, 210 in lung and 213 in PBMC. These genes showed differential expression (DE) patterns on analysis by visualization and integrated discovery (DAVID). The DE genes involved in the immune response included genes related to the inflammatory response (CD163), the innate immune response (TLR2, TLR4, MYD88, TIRAP), cell adhesion (CD34, SELE, SELL, SELP, ICAM-1, ICAM-2, VCAM-1), antigen processing and presentation (MHC protein complex) and angiogenesis (VEGF), together with genes encoding cytokines (interleukins). Five selected genes were validated by qRT-PCR analysis.ConclusionsWe studied the response to infection with S. suis 2 strain SC19 by microarray analysis. Our findings confirmed some genes identified in previous studies and discovered numerous additional genes that potentially function in S. suis 2 infections in vivo. This new information will form the foundation of future investigations into the pathogenesis of S. suis.
Highlights
IntroductionS suis 2 infections have high mortality rates and are characterized by meningitis, septicemia and pneumonia
Streptococcus suis serotype 2 (S. suis 2) is an important pathogen of pigs
There were 617 genes were changed in the lung, 293 up-regulated and 324 downregulated and 685 genes were changed in peripheral blood mononuclear cells (PBMC), 403 upregulated and 282 down-regulated
Summary
S suis 2 infections have high mortality rates and are characterized by meningitis, septicemia and pneumonia. S. suis 2 is an emerging zoonotic agent and can infect humans that are exposed to pigs or their by-products. To increase our knowledge of the pathogenesis of meningitis, septicemia and pneumonia in pigs caused by S. suis 2, we profiled the response of peripheral blood mononuclear cells (PBMC), brain and lung tissues to infection with S. suis 2 strain SC19 using the Affymetrix Porcine Genome Array. Streptococcus suis (S. suis 2) is an important pathogen of pigs that causes high mortality and is responsible for considerable economic loss to the porcine industry [1]. S. suis 2 is an emerging zoonotic agent and has been isolated from a wide range of mammalian species, including humans, who are often infected via skin wounds during contact with pigs and their products [3]. In Hong Kong, infection with S. suis is the third most common cause of community-acquired bacterial meningitis, and in Vietnam it is the leading cause of meningitis in adult humans [9]
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