Understanding Host-Pathogen Interactions With the Use of Galleria mellonella

Abstract

The <em>Galleria mellonella</em>; the larvae of the Greater Wax Moth, is a safe and effective means to study host-pathogen interactions in the undergraduate setting. The use of animal models in the teaching classroom provides an opportunity to discuss proper animal use and the benefits of alternative model systems in research. In this lesson, we developed a laboratory experience for students to learn about the use of <em>G. mellonella </em>as a model to study<em> </em>bacterial virulence. Students evaluate the virulence of a suite of microbes by injecting larvae and analyzing their survival over time. Students gain the opportunity to reinforce basic microbiology techniques such as aseptic technique and bacterial enumeration, while learning about new methods involving animal use in research. Students learn how to report their data using survival plots. More importantly, this lesson gives students a hands-on experience working with an <em>in vivo</em> system and complements discussions of innate immunity and bacterial virulence by providing a visual and quantitative approach to the study of virulence. <em>Primary image:&nbsp;</em>Uninfected Greater wax moth larvae (<em>Galleria&nbsp;mellonella</em>) on petri dishes.