Abstract
We have identified fifty-eight samples that were positive for Dengue-4 among 119 samples with negative diagnoses for dengue via the Platelia™ dengue NS1 Ag in Aracaju, State of Sergipe, Brazil. We determined that the low sensitivity of the NS1 Ag test could be related to secondary dengue infections in the studied population. Therefore, we concluded that the sensitivity and specificity of the Platelia™ dengue NS1 Ag test as a screening method for monitoring circulating dengue serotypes must be reevaluated. In addition, regional endo-epidemic profiles should also be considered due to the prevalence of secondary responses.
Highlights
Dengue is considered the most important of the arboviruses affecting humans
The DENV-4 serotype was detected for the first time in Brazil in 1982 during a dengue outbreak in the north of the country, and since no new cases of DENV-4 were detected until 2010 [6]
In Sergipe, DENV-4 was not reported before March 2012, but, when we analyzed blood samples that were negative for dengue by the PlateliaTM NS1 Ag test collected between September 2011 and February 2012, we found that 48.7% were positive for DENV-4 by semi-nested RT-PCR
Summary
Dengue is considered the most important of the arboviruses affecting humans. It is transmitted mainly by the mosquito Aedes aegypti and has four known serotypes: DENV-1, DENV-2, DENV-3 and DENV-4. Patients arriving for care within five days of the onset of disease symptoms are screened for viral isolation in health care sentinel units and, provide early detection of circulating serotypes in a given area [7]. Patients were asked and provided their oral consent to enter in the Ministry of Health protocol of diagnosis and isolation of serotype dengue virus.
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