Abstract
Eukaryotes have at least three nuclear, DNA-dependent RNA polymerases (Pols) responsible for synthesizing all the genome-encoded RNA required by the cell. Since the discovery of the Pols, their characterization has been executed under disparate experimental conditions, making direct comparisons of the Pols challenging. To that end, we have devised a method whereby the Pols can be directly compared on a millisecond time scale using a chemical-quenched flow instrument and a versatile, promoter-independent, in vitro transcription assay.
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