Uncovering the synergistic interplay of melatonin and spermidine in the alleviation of nematode stress in Solanum lycopersicum

Abstract

Worldwide, biotic stress severely degrades agricultural output and increases the risk of starvation. Root-knot nematodes (Meloidogyne incognita) are one of the important endoparasites that adversely affect the growth and development in plants, thus affecting their productivity. Contrarily, humans employ a number of unfriendly techniques, such as chemical applications, to manage biotic stressors. Use of Plant Growth Regulators is an environmentally safe alternative method against chemical pesticides that can be used to defend plants from biotic stressors. Melatonin and polyamines have been broadly found in multiple physiological processes and in diverse biotic and abiotic stresses faced by plants. In the contemporaneous study, we conducted an in vitro experiment which disclosed that pretreated seeds with melatonin and spermidine (a polyamine), decreased root galls in afflicted plants and uplifted the growth of Solanum lycopersicum seedlings. According to our findings, tomato plants' photosynthetic efficiency dropped and reactive oxygen species levels dramatically rose after nematode inoculation. On the other hand, melatonin and spermidine decreased oxidative stress by scavenging hydrogen peroxide and decreased malonaldehyde. The present work investigated improvement in growth characteristics, photosynthetic pigments, antioxidative enzymes and non-antioxidative enzymes in PGR treated tomato seedlings even during the nematode stress. Confocal studies evaluated nuclear damage within root apices and intensity of blue colour was directly proportional to nuclear damage. The findings of the present investigation support the use of plant growth regulators, melatonin and spermidine as seed priming agent to manage nematode stress in plants.