Uncovering the hidden hosts: Identifying inoculum reservoirs for Phytophthora pseudosyringae in Nothofagus forests in Chile

Abstract

AbstractMortality of Nothofagus trees in native forests in Chile has been observed for more than 30 years. Phytophthora pseudosyringae was identified as the causal agent of partial defoliation and bleeding cankers on Nothofagus obliqua and N. alpina. Nevertheless, to improve the conservation of natural resources it is crucial to determine potential native hosts that may act as inoculum reservoirs. Two N. obliqua stands were visited and all native plant species with symptoms resembling those caused by Phytophthora spp. were examined. Seven isolates from cortical and foliar tissues were isolated and subsequentially identified as P. pseudosyringae. Pathogenicity tests were carried out on eight species from a native forest. P. pseudosyringae caused cankers in N. obliqua, N. dombeyi and Persea lingue. Under natural conditions, no symptoms were detected on leaves of Cryptocarya alba, N. dombeyi, N. obliqua or Peumus boldus, but lesions were formed in inoculation assays under a controlled environment, suggesting that these species may act as hosts. Leaf necrosis in P. lingue was observed in both natural and controlled conditions. P. pseudosyringae can sporulate on lesions of C. alba, N. dombeyi and N. obliqua leaves. In Sophora macrocarpa, sporulation was observed both on asymptomatic tissues and on lesions. The frequent association of S. macrocarpa in the understorey of Nothofagus spp. strengthens the putative role of S. macrocarpa as an inoculum reservoir for epidemic events in Nothofagus. This is the first study carried out in the Southern Hemisphere on the life cycle of P. pseudosyringae in native Nothofagus forests.