Uncoupling mitochondrial activity maintains body [formula omitted] during hemorrhage-induced O2 deficit in the anesthetized rat

Abstract

During a hemorrhagic shock (HS), O2 uptake (V˙O2) decreases as soon as the rate of O2 delivery (DO2) drops below a “critical level”, a response accounted for by the reduction in mitochondrial O2supply. In urethane-anesthetized rats, DO2 was decreased within 20min from 21.5 to 2.8mlmin−1 by slowly withdrawing 18mlkg−1 of blood. This led to a reduction in V˙O2 from 6.1 to 2.4mlmin−1 (n=5, p<0.01). Decoupling mitochondrial oxidative activity by injecting 2,4-DNP (6mgkg−1, iv) before HS elevated V˙O2 to 11.9±1.2mlmin−1 (n=6, p<0.01), which remained above control HS values throughout most of the hemorrhage. This was associated with higher levels of O2 extraction, cardiac output and ventilation than in control HS. DO2–V˙O2 relationship was shifted upward and to the left following DNP. In conclusion, cellular and systemic mechanisms, decreasing O2demand, account for a large part of HS induced V˙O2 decline resulting in an additional reduction in DO2.