Abstract
Abstract Tryptic digest of Lysozyme were analyzed by matrix-assisted laser desorption ionization tandem time of flight-mass spectrometry (MALDI-TOF/TOF-MS). Six unique peptides were identified with high confidence by database searching and the Mascot score is 420 with the protein coverage of 54%. Furthermore, several uncommon modifications including deamidation of Asn residue, deamidation of Asn plus oxidation of Met, deamidation of Asn plus oxidation of Met and oxidation of Trp were observed for peptide IVSDGNGMNAWVAWR (98→112). Furthermore, a dehydration reaction was observed in the process of MALDI for this peptide. All above results showed that the Asparagine at 103 rd position has a highly chemical reactivity. In addition, the modification of propionamide (Cystine) was observed for another two peptides. This study indicated that, for those of unmatched data by database searching, the data utilization and the certainty of analysis result would be increased by using manual explanation and selecting very low abundant ions in primary spectrum. This method is also helpful for finding potential post-translational modifications.
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