Abstract
Abstract The results of a collaborative study are reported. Nine collaborators from industry and government analyzed 3 samples each. The intermediates were separated from FD&C Red No. 40 and from each other on a cellulose column, using 40 and 20% aqueous ammonium sulfate as eluants, and were quantitated by UV spectrometry. Cresidinesulfonic acid, Schaeffer’s salt, and 6,6′-oxybis(2-naphthalenesulfonic acid) were recovered at an average level of 99.7%. Between-laboratory standard deviations ranged from 0.007 to 0.072% when the intermediates were present at levels of 0.03 to 1.00%. The method has been adopted as official first action.
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