Abstract
Cell counting is a fundamental procedure in living cell culture-based experiments and protocols in which the cell number quantification is required. The number of cells is one of the parameters necessary to investigate several cell culture features requiring to be monitored as function of time, such as cell viability, proliferation, growth, fitness and metabolism. Aim of this paper is contributing to declare a comprehensive uncertainty budget for cell counting through metabolic assays according to the EURACHEM/CITAC Guide Quantifying Uncertainty in Analytical Measurement.
Highlights
The aim of this paper is to contribute to declare a comprehensive uncertainty budget for cell counting by metabolic assays according to the EURACHEM/CITAC Guide Quantifying Uncertainty in Analytical Measurement [1].The lack of traceability of diagnostic and clinical measurement justifies the consideration on the quantification of cells number as a measurement service requested by National Metrology Institutes (NMIs)
Cell counting is a fundamental procedure in living cell culture-based experiments and protocols in which the cell number quantification is required
The cell number is one of the parameters necessary to investigate several cell culture features such as cell viability, proliferation, growth, fitness and metabolism which require a monitoring as function of time [3]
Summary
- Observations on the worst case uncertainty Laura Fabbiano, Nicola Giaquinto, Mario Savino et al. - Uncertainty analysis of a stereo system performing ego-motion measurements in a simulated planetary environment M Pertile, S Debei and E Lorenzini. - The evaluation of uncertainty in mass calibration: possible approaches in a comparison study J A Sousa, A B Forbes, A S Ribeiro et al. This content was downloaded from IP address 193.204.114.64 on 02/02/2021 at 17:44
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