Abstract
It is shown that the modified sequential simplex algorithm can be used as the basis for the unattended optimisation of reversed-phase liquid chromatographic separations. A chromatographic response function is computed to evaluate individual chromatograms with respect to resolution and analysis time. This function is used automatically by a microprocessor-controlled chromatograph to optimise experimental parameters. The use of the procedure is illustrated by the automated optimisation of two and three component mobile phases and the optimisation of a gradient elution profile.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
