Abstract
Reconstructive surgery of congenital heart disease (CHD) remains inadequate due to the inability of prosthetic grafts to match the somatic growth of pediatric patients. Functionalization of grafts with mesenchymal stem cells (MSCs) may provide a solution. However, MSCs represent a heterogeneous population characterized by wide diversity across different tissue sources. Here we investigated the suitability of umbilical cord pericytes (UCPs) in neonatal vascular engineering. Explant outgrowth followed by immunomagnetic sorting was used to isolate neural/glial antigen 2 (NG2)+/CD31− UCPs. Expanded NG2 UCPs showed consistent antigenic phenotype, including expression of mesenchymal and stemness markers, and high proliferation rate. They could be induced to a vascular smooth muscle cell-like phenotype after exposure to differentiation medium, as evidenced by the expression of transgelin and smooth muscle myosin heavy chain. Analysis of cell monolayers and conditioned medium revealed production of extracellular matrix proteins and the secretion of major angiocrine factors, which conferred UCPs with ability to promote endothelial cell migration and tube formation. Decellularized swine-derived grafts were functionalized using UCPs and cultured under static and dynamic flow conditions. UCPs were observed to integrate into the outer layer of the graft and modify the extracellular environment, resulting in improved elasticity and rupture strain in comparison with acellular grafts. These findings demonstrate that a homogeneous pericyte-like population can be efficiently isolated and expanded from human cords and integrated in acellular grafts currently used for repair of CHD. Functional assays suggest that NG2 UCPs may represent a viable option for neonatal tissue engineering applications.
Highlights
Congenital heart disease (CHD) is the most prevalent congenital abnormality, affecting ∼1% of newborns globally, with incidence rate seemingly increasing over the last 50 years [1]
Immunohistochemical analysis of the umbilical cord confirmed no CD31−/CD34+ pericytes were present within the tissue; only sporadic staining of neural/glial antigen 2 (NG2) umbilical cord pericytes (UCPs) was detected (Figure 2B)
We found that endothelial cells (ECs) incubated with NG2+ umbilical cord pericyte (NG2 UCP) CM migrated four times farther than Human umbilical vein endothelial cells (HUVECs) incubated with either unconditioned medium or mesenchymal stem cells (MSCs) CM (Figures 6I,J)
Summary
Congenital heart disease (CHD) is the most prevalent congenital abnormality, affecting ∼1% of newborns globally, with incidence rate seemingly increasing over the last 50 years [1]. Prognosis has significantly improved, CHD remains one of the primary causes of perinatal mortality, accounting for over 250,000 deaths in 2017 [2]. Complex malformations such as Tetralogy of Fallot (ToF) often require immediate surgical correction using a prosthetic graft. In recent years, engineering of prosthetic grafts with regenerative cells, such as mesenchymal stem cells (MSCs), has emerged as a potential solution [5, 6]. An alternative cell population from an accessible tissue, such as the umbilical cord, would offer obvious advantages over cardiac pericytes
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
