Abstract
Abstract A simple and rapid procedure for the determination of dimetridazole (l,2-dimethyl-5-nitroimidazole) in feeds is described, using only liquid-liquid extractions before ultraviolet (UV) measurement. The drug is more effectively separated from interfering substances by this method than by the 2 alumina chromatographic steps required in the official first action AOAC method. Dimetridazole is extracted from feed with chloroform and trisodium phosphate solution and transferred from chloroform into hydrochloric acid; sodium hydroxide is added to the solution and the drug is transferred into benzene for UV measurement. Ipronidazole and zoalene interfere. The presence of bentonite decreases recovery. The interference from natural feed ingredients is negligible. An average coefficient of variation of 1.9% and an average recovery of 98% were found on repetitive assays of 3 feeds of known dimetridazole content.
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