Abstract
The use of resonance Raman spectroscopy for the identification of microorganisms began with the selective detection of carotenoids in chromobacteria. In that work, it was shown that the carotenoid spectra excited at 488 nm were markedly different for different organisms. Somewhat later a more detailed study showed that major differences in peak heights and peak height ratios allow identification on the species level. Most information is contained in the 900–1600 cm−1 region. All chromobacteria exhibited two very intense bands associated with carbon-carbon single-bond and double-bond stretching vibrations of the conjugated carotenoid chains at around 1150 cm−1 and 1500 cm−1, respectively.
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