Abstract
A novel ultraviolet light irradiation (UV-C, 254 nm) process was designed as an additional safety feature for manufacturing of spray dried porcine plasma (SDPP). In Exp. 1, three 10-L batches of bovine plasma were inoculated with 105.2±0.12 tissue culture infectious dose 50 (TCID50) of porcine parvovirus (PPV) per mL of plasma and subjected to UV-C ranging from 0 to 9180 J/L. No viable PPV was detected in bovine plasma by micro-titer assay in SK6 cell culture after UV-C at 2295 J/L. In Exp. 2, porcine plasma was subjected to UV-C (3672 J/L), then spray dried and mixed in complete mash diets. Diets were a control without SDPP (Control), UV-C SDPP either at 3% (UVSDPP3) or 6% (UVSDPP6) and non-UV-C SDPP at 3% (SDPP3) or 6% (SDPP6). Diets were fed ad libitum to 320 weaned pigs (26 d of age; 16 pens/diet; 4 pigs/pen) for 14 d after weaning and a common diet was fed d 15 to 28. During d 0 to 14, pigs fed UVSDPP3, UVSDPP6, or SDPP6 had higher (P < 0.05) weight gain and feed intake than control. During d 0 to 28, pigs fed UVSDPP3 and UVSDPP6 had higher (P < 0.05) weight gain and feed intake than control and SDPP3, and SDPP6 had higher (P < 0.05) feed intake than control. Also, pigs fed UVSDPP had higher (P < 0.05) weight gain than pigs fed SDPP. In conclusion, UV-C inactivated PPV in liquid plasma and UVSDPP used in pig feed had no detrimental effects on pig performance.
Highlights
In Exp. 1, three 10L batches of bovine plasma were inoculated with 105.2±0.12 tissue culture infectious dose 50 (TCID50) of porcine parvovirus (PPV) per mL of plasma and subjected to UV-C ranging from 0 to 9180 J/L
Diets were a control without spray dried porcine plasma (SDPP) (Control), UV-C SDPP either at 3% (UVSDPP3) or 6% (UVSDPP6) and non-UV-C SDPP at 3% (SDPP3) or 6% (SDPP6)
Neutralizing antibodies to PPV were not detected in the bovine plasma used in Exp. 1
Summary
Ethic StatementPigs used in experiment 2 were housed in compliance with the Directive 2010/63/EU of the European parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes. The zootechnical procedures used in this experiment (i.e. weighing) were carried out by experienced and authorized personnel and were not susceptible for causing suffering to the animals. A reactor typically consists of a stainless steel inlet and outlet chamber with a stainless steel corrugated spiral tube between the chambers. Inside the spiral tube is an UV-C 254 nm wavelength germicidal lamp of 100 Watt (W) output (30 W UV-C output) which is protected by a quartz sleeve. The liquid flows between the corrugated spiral tube and the quartz sleeve. The liquid is pumped from the inlet chamber into the actual reactor, the gap between the quartz sleeve, and the corrugated spiral tubing at a minimum flow rate of 3800 L/h with a Reynolds value in excess of 7500, indicating turbulent flow
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