Abstract
Flavonoids and polyphenols from the strawberry and other fruits have been proposed to reduce the oxidative stress produced by the obesity and her complications. Moreover, it has been proposed that irradiation with UV‐C to strawberry may increase the antioxidant capacity of this fruit. The aim of the present study was to explore the effects of the UV‐C on antioxidant capacity of strawberry in vitro and in vivo. Strawberry slices were irradiated with ultraviolet light‐C (UV‐C) at 1.2 W/m2/16.5 min; then, the power antioxidant was isolated from the nonirradiated and irradiated strawberry slices into an organic phase, which was lyophilized to finally producing a nonirradiated strawberry extract (NSE) and UV‐irradiated strawberry extract (UViSE) powder. After the antioxidant capacity of both extracts were determined in vitro using the Trolox equivalent antioxidant capacity (TEAC) assay and in vivo using high‐fat diet‐induced obese rats. Our results demonstrated that irradiation with UV‐C to strawberry slices increased the antioxidants content, which was corroborated in vitro, where the antioxidant capacity of UViSE was higher than the NSE. However, in obese rats, the reduction in the oxidative damage by the UViSE and NSE were similar in peripheral tissues. Interestingly, the UViSE was better than the NSE to reduce the oxidative damage in brain. In conclusion, UV‐irradiation increases the antioxidants content of strawberry that is correlated with an increased antioxidant capacity in vitro, but in rats, this antioxidant capacity may be more effective in brain than in peripheral tissues.
Highlights
Sedentary lifestyle and unhealthy food habits are considered the main culprits of obesity (Han, Lawlor, & Kimm, 2010)
We observed that the antioxidant capacity in vitro was significantly increased in the ultraviolet light (UV)-irradiated strawberry extract (UViSE) compared with the nonirradiated strawberry extract (NSE) (1492 vs. 1035.7 nmol/L of Trolox equivalent (TE)/100 g of dry weight, p = .001)
Once we demonstrated that ultraviolet light-C (UV-C) radiation increases the antioxidant capacity in vitro; we determined whether this antioxidant capacity occurs in vivo; for which, we feed to rats with high- fat diet (HFD)
Summary
It has been demonstrated that dietary supplementation with the antioxidant curcumin reduces oxidative stress (Martínez-Morúa et al, 2013), and reduces the brain damage by increasing the brain derived neurotrophic factor (BDNF) levels in obesity and diabetes mice (Franco-Robles et al, 2014). With respect to UV radiation, Ayala and others described that this irradiation increases the concentration of phenolic compounds and various antioxidants in strawberries (Ayala Gil & Lozoya Gloria, 2016). In the present study, the aim was to produce extract of UV- irradiated strawberry and to determine his effect on the oxidative stress in HFD-fed rats
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