Abstract
The present official method for the determination of diethylstilbestrol is not specific, and incomplete extraction of active material from small dosage tablets is often encountered. To circumvent these difficulties, two new methods of assay have been developed. The procedures depend on photochemical changes which diethylstilbestrol undergoes when it is irradiated with short wavelength ultraviolet light and on the reproducible shift of the ultraviolet absorption spectrum of diethylstilbestrol in acid and in alkaline solution. Apparatus for the irradiation method includes a suitable strong ultraviolet light source and a spectrophotometer capable of measuring visible light absorption, while the ultraviolet absorptiometric method requires a spectrophotometer operating in the ultraviolet region. Interferences in the irradiation procedure are primarily inhibitory in nature while those in the UV absorptiometric method are those compounds which exhibit a shift in absorption spectrum in the 280 mμ range in acid and alkaline solution. The nature of the pigment produced by irradiation is not known.
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