Abstract
Ultraviolet irradiation of the single-stranded Coliphage R17 leads to inactivation of the invitro messenger activity of its RNA. Uridine-photohydration is the major reaction occurring in the RNA under these conditions. At a residual activity of the synthetase gene of 37 per cent, there are 11.2 uridine-photohydrates present in the total R17 genome, while only 5.3 photohydrates are necessary to decrease the translation of the coat gene to the same value. Denovo incorporation of histidine into the coat protein is observed with R17-RNA from ultraviolet-irradiated phage, indicating that uridine-photohydrates can miscode as cytidine in an invitro translation system.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
