Abstract
Ultraviolet difference spectral data provided useful information to differentiate the binding modes between antitumor-active and -inactive platinum complexes. The uv difference spectra for the DNA samples treated with platinum complexes exhibit a band at 295 nm due to the change in electron distribution of nucleic acid bases and a band at 270 nm due to the change in the secondary structure of DNA. The absorption ratio, ΔA 270/ΔA 295, is an index for the change of the secondary structure of DNA induced by binding with platinum. Changes in the DNA secondary structure induced by antitumor-active platinum complexes are greater than those induced by antitumor-inactive platinum complexes, and the uv difference spectral behavior of antitumor-active platinum complexes is similar. A tendency for preferential binding toward the guanine residues in DNA was confirmed in this study. Melting profiles for the DNA samples treated with platinum complexes showed a decrease in hyperchromicity and a broadening of transition width. The platinum complexes, other than trans-Pt(NH 3) 2Cl 2, decreased the melting temperature.
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