Ultraviolet and Fluorimetric Determination of Monosubstituted Acetylenic Hypisotics After Conversion to Their Cohresponding Carbonyl Copmpounds

Abstract

Abstract Monosubstituted acetylenic hypnotics; ethchlorvynol, ethinamate and methylpentynol carbamale can be determned spectrophotometrically, after conversion to their α, β -unsaturated carbonyl compounds. The latter exhibit intense absorption spectra at 268 nm, 240 nm and 237 nm, for ethchlorvynol, ethinamate and methylpentynol carbamate respectively. Moreover, these carbonyl compounds contain active methylene groups which react with N1-methylnicotinamide iodide, in presence of alkali. On addition of formic acid, an intense fluorescence io obtained; by measuring this fluorescence, as little as 5–50 μg of acetylenic hypnotics can be determined with good accuracy.