Abstract
Received 8 July 1970 1. Introduction One of the criteria of purity of a preparation of RNA polymerase (EC 2.7.7.6) is its 280/260 rq~ absorbance ratio. Enzyme preparations with a ratio below 1.65 are assumed to contain some contami- nating nucleic acids or their derivatives [l] . How- ever, no nucleic acid was detected in a purified en- zyme preparation of absorbance ratio 1.25 [2], either by the Dische method (limit of detection 1%) or the orcinol procedure (limit of detection 0.6%). From the data of Warburg and Christian [3] ,
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