Abstract
Rat superior cervical ganglion fixed with osmium tetroxide-zinc iodide (ZIO) reagent was examined in the electron microscope. The 40 nm diameter vesicles of cholinergic synaptic terminals were found to contain ZIO-reactive material, but the accompanying 90 nm dense-cored synaptic vesicles were ZIO-negative. The catecholamine-containing granules of chromaffin cells contained ZIO-reactive material and in addition ZIO-positive granules 30–50 nm in diameter were observed in the adrenergic postganglionic cell bodies. The specificity of the ZIO reagent is discussed in the light of these findings.
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