Ultrastructure of the reproductive system of the black swamp snake (Seminatrix pygaea). IV. Occurrence of an ampulla ductus deferentis.

Abstract

The distal end of the ductus deferens of Seminatrix pygaea (Cope) differs from more proximal portions of the tube by possessing a highly fluctuated epithelium. Similar morphology has been reported in this area in one lizard and some mammals and the region has been designated in these taxa as the ampulla ductus deferentis. In this study, light and transmission electron microscopy were used to study the posterior ductus deferens in S. pygaea and compare the histology in this region to more proximal regions of the duct and to descriptions of the ampulla of other species. Seminatrix pygaea stores sperm throughout the ductus deferens during the entire year. The epithelium of the ductus deferens is not secretory, but the presence of numerous small apical vesicles indicates a role in fluid absorption. In addition to the highly folded epithelium, clusters of sperm nuclei are more intimately associated with the apical ampullary epithelium than elsewhere in the ductus deferens. No evidence of phagocytosis of sperm was found. In contrast, the ampulla of mammals and presumably the lizard Calotes versicolor is glandular and phagocytic. The common character shared by the squamates and mammals with specializations in this area is the folded epithelium, and whether this is due to shared ancestry with amniotes or is homoplastic is unresolved.