Abstract
First and second year overwintering green leaves of Taxus canadensis were collected during February from plants in Lowell, Massachusetts. This month is usually the coldest time of the year, and with the ground frozen, water is unavailable for these plants. Leaves were sectioned into 2-3 mm lengths and fixed in 5% glutaraldehyde in 0.075 M NaOH-PIPES buffer immediately upon collection. The tissues were then post-fixed in 2% OsO4 and dehydrated in ethanol and embedded in Spurr's resin (Spurr, 1969).
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