Ultrastructure of Spermiogenesis and the Spermatozoon of Anoplocephaloides dentata (Cestoda, Cyclophyllidea, Anoplocephalidae), an Intestinal Parasite of Arvicolidae Rodents

Abstract

Spermiogenesis in Anoplocephaloides dentata begins with the formation of a differentiation zone delimited by a ring of arched membranes. This conical area shows 2 parallel centrioles with associated anterior reduced striated roots but without an intercentriolar body. Only 1 of the centrioles develops an axoneme that grows into a cytoplasmic extension. Two crestlike bodies appear when the nucleus initiates its migration along the spermatid body. We describe for the first time at the end of spermiogenesis the formation of an apical cone before the strangulation of the ring of arched membranes. The mature spermatozoon of A. dentata is filiform, tapered at both ends, and lacks mitochondria. Its anterior extremity has an apical cone measuring about 1,400 x 350 nm and 2 crestlike bodies. Cortical microtubules are spiralled at an angle of about 30 degrees to the spermatozoon axis. The axoneme, of the 9+1 pattern of Trepaxonemata (Polycladida, Seriata, "Typhloplanoida," "Dalyellioida," and Neodermata lacks a periaxonemal sheath and does not reach the extremities of the spermatozoon. Numerous granules of electron-dense material are observed in the posterior regions of each spermatozoon. Analysis of ultrastructural features found during spermiogenesis in A. dentata corroborates the presence of striated roots associated with the centrioles in cyclophyllidean species. Moreover, the presence of striated roots is described for the first time in type IV spermiogenesis.