Ultrastructure of spermatogenesis of Taenia taeniaeformis (Batsch, 1786) (Cestoda, Cyclophyllidea, Taeniidae) and comparison of spermatological characters in the family Taeniidae Ludwig, 1886

Abstract

AbstractThe ultrastructure of spermatogenesis of Taenia taeniaeformis is described for the first time by means of transmission electron microscopy (TEM). Mature testes contain all stages of spermatogenesis; primary spermatogonia are usually situated at the periphery and mature spermatozoa in the centre of testes. The general process is similar to that described in other cestodes. Six incomplete, synchronic cytokineses occur: four mitotic and two meiotic cell divisions. All these divisions occur simultaneously, resulting in a rosette cluster of four tertiary spermatogonia, then eight quaternary spermatogonia, and subsequently sixteen primary spermatocytes. All of these enter into a growth period and their enlarged nuclei move to the periphery of cells of the rosettes. The first meiotic division forms thirty-two secondary spermatocytes and after the second meiotic division, there are sixty-four spermatids. Spermiogenesis in T. taeniaeformis corresponds to the Ba and Marchand’s Type 3 and begins with the formation of a differentiation zone in the form of a conical projection of cytoplasm delimited by a ring of arching membranes and surrounded by submembranous cortical microtubules. Within this area, there are two centrioles, orthogonally disposed, and vestigial striated rootlets. Only one of the centrioles develops a flagellum that grows externally to the cytoplasmic extension. Posteriorly, a flagellar rotation inferior to 90° occurs and the flagellum becomes parallel to the cytoplasmic extension. Later, the two processes fuse during the so-called proximodistal fusion. The nucleus elongates and moves into the cytoplasmic extension. In the final stage of spermiogenesis, a single crested body appears at the base of the differentiating spermatozoon. Finally, the ring of arching membranes constricts and the young spermatozoon detaches from the residual cytoplasm. Ultrastructural aspects of spermatogenesis are compared with that of other cestodes studied to date, particularly of the family Taeniidae.